Analysis on characteristic of stage Ⅰ occupational cement pneumoconiosis patients / 中华劳动卫生职业病杂志

Objective: To analyze the clinical and imaging characteristics of stage Ⅰ occupational cement pneumoconiosis patients. Methods: In October 2021, the data of patients with occupational cement pneumoconiosis diagnosed by the Third Hospital of Peking University from 2014 to 2020 were collected, and the data of the patients' initial exposure age, dust exposure duration, diagnosis age, incubation period, chest X-ray findings, lung function and other data were analyzed retrospectively. Spearman grade correlation was used for correlation analysis of grade count data. The influencing factors of lung function were analyzed by binary logistic regression. Results: A total of 107 patients were enrolled in the study. There were 80 male patients and 27 female patients. The inital exposure age was (26.2±7.7) years, the diagnosis age was (59.4±7.9) years, the dust exposure duration was (17.9±8.0) years, and the incubation period was (33.1±10.3) years. The initial dust exposure age and the dust exposure duration in female patients were less than those in men, and the incubation period was longer than that in men (P<0.05). The imaging analysis showed the small opacities as"pp"accounted for 54.2%. 82 patients (76.6%) had small opacities distributed in two lung areas. The lung areas distribution of small opacities in female patients was less than that in male patients (2.04±0.19 vs 2.41±0.69, P<0.001). There were 57 cases of normal pulmonary function, 41 cases of mild abnormality and 9 cases of moderate abnormality. The number of lung regions with small opacities on X-ray was the risk factor for abnormal lung function in cement pneumoconiosis patients (OR=2.491, 95%CI=1.197-5.183, P=0.015) . Conclusion: The patients with occupational cement pneumoconiosis had long dust exposure duration and incubation period, light imaging changes and pulmonary function damage. The abnormal lung function was related to the range of pulmonary involvement.

Interval training effects on cardiorespiratory capacity of swimmers / Efeito do treinamento intervalado sobre a capacidade cardiorrespiratória de nadadores / Efecto del entrenamiento por intervalos en la capacidad cardiorrespiratoria de nadadores

ABSTRACT Introduction: Cardiopulmonary function is directly related to the body's ability to perform metabolism under aerobic conditions. It is a key characteristic for the training of professional swimmers. It is believed that adding interval training to traditional workouts may contribute to the aerobic endurance of these athletes. Objective: Evaluate the interval training effects on the cardiorespiratory capacity of swimmers. Methods: Twenty volunteer swimmers were randomly divided into two groups for seven weeks. There were 4 hours of class per week, totaling 28 hours of training. In the formal training stage, the experimental group performed intermediate-intensity training, while the control group continued to perform traditional swim team training. The experimental indicators pertinent to the evaluated athletes were collected, tested, and analyzed before and after the experiment. Results: The VO2 of the athletes in the experimental group increased from 3.90±0.67L/min before the experiment to 4.06±0.73L/min after the experiment; VE increased from 156.08±35.76L before the experiment to 180.08±39.42L after the experiment; underwater endurance time increased from 46.80±8.43s before the experiment to 55.49±7.60s after the experiment. Conclusion: Intermittent training improves athletes' physical fitness and sports capacity, being an effective method to improve cardiopulmonary function. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: A função cardiopulmonar está diretamente relacionada à capacidade do organismo de executar a metabolização sob condições aeróbicas. É uma característica chave para os treinamentos de nadadores profissionais e acredita-se que a adição do treinamento intervalado nos treinos tradicionais possa contribuir à resistência aeróbica desses atletas. Objetivo: Avaliar o efeito do treinamento intervalado sobre a capacidade cardiorrespiratória dos nadadores. Métodos: Vinte nadadores voluntários foram divididos aleatoriamente em dois grupos durante 7 semanas. Foram 4 horas de aula por semana, totalizando 28 horas de treino. Na etapa de treinamento formal, o grupo experimental realizou essencialmente o treinamento de intensidade intermediária, enquanto o grupo de controle permaneceu executando o treinamento tradicional da equipe de natação. Os indicadores experimentais pertinentes aos atletas avaliados foram coletados, testados e analisados antes e depois do experimento. Resultados: O VO2 dos atletas do grupo experimental aumentou de 3,90±0,67L/min antes do experimento para 4,06±0,73L/min após o experimento; o VE aumentou de 156,08±35,76L antes do experimento para 180,08±39,42L após o experimento; o tempo de resistência subaquática aumentou de 46,80±8,43s antes do experimento para 55,49±7,60s após o experimento. Conclusão: O treinamento intermitente contribui para melhorar a aptidão física e a capacidade esportiva dos atletas, sendo um método eficaz no aprimoramento da função cardiopulmonar. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: La función cardiopulmonar está directamente relacionada con la capacidad del organismo para realizar el metabolismo en condiciones aeróbicas. Es una característica clave para los entrenamientos de los nadadores profesionales y se cree que la adición del entrenamiento interválico en los entrenamientos tradicionales puede contribuir a la resistencia aeróbica de estos atletas. Objetivo: Evaluar el efecto del entrenamiento por intervalos sobre la capacidad cardiorrespiratoria de los nadadores. Métodos: Veinte nadadores voluntarios fueron divididos aleatoriamente en dos grupos durante 7 semanas. Se impartieron 4 horas de clase a la semana, totalizando 28 horas de entrenamiento. En la etapa de entrenamiento formal, el grupo experimental realizó esencialmente un entrenamiento de intensidad intermedia, mientras que el grupo control permaneció realizando el entrenamiento tradicional del equipo de natación. Los indicadores experimentales pertinentes a los atletas evaluados fueron recogidos, testados y analizados antes y después del experimento. Resultados: El VO2 de los atletas del grupo experimental aumentó de 3,90±0,67L/min antes del experimento a 4,06±0,73L/min después del experimento; el VE aumentó de 156,08±35,76L antes del experimento a 180,08±39,42L después del experimento; el tiempo de resistencia subacuática aumentó de 46,80±8,43s antes del experimento a 55,49±7,60s después del experimento. Conclusión: El entrenamiento por intervalos contribuye a mejorar la forma física y la capacidad deportiva de los atletas, siendo un método eficaz para mejorar la función cardiopulmonar. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Activating effect of extracellular histones on heparinase in chlorine-induced acute lung injury / 中国职业医学

OBJECTIVE: To investigate the induction and activation of heparinase by extracellular histones in acute respiratory distress syndrome(ARDS) induced by chlorine in mice.METHODS: The specific pathogen free adult male C57 BL/6 mice were randomly divided into control group, chlorine injured group, histone injured group, anti-histone antibody group and heparinase inhibitor group, with six mice in each group.The mice in the control group and histone injured group were exposed to clean air, and the mice in the other three groups were exposed to chlorine gas at a dose of 580.0 mg/m~3 for 30 minutes by systemic dynamic inhalation.Mice in the histone injured group were injected with 50 mg/kg body weight calf thymus histone by tail vein.One hour before exposure, mice in the anti-histone antibody group were pretreated with 20 mg/kg body weight anti-histone H4 antibody by tail vein injection, and mice in the heparinase inhibitor group were injected with 2 mg/kg body weight OGT2115(heparinase inhibitor). The other three groups were given equal volume of 0.9% sodium chloride solution by tail vein injection. After 24 hours of exposure, arterial blood was collected for blood gas analysis and the lung tissue was collected for histopathological examination. The protein level of heparinase in lung tissue were detected using enzyme-linked immunosorbent assay, and the activity of heparinase were detected by measuring the product of heparan degradation. The protein expression of pro-heparinase and active heparinase were detected by Western blotting.RESULTS: The dyspnea developed of mice in the chlorine injured group and histone injured group, diffuse inflammation occurred in lung tissue, the oxygenation index in arterial blood decreased(all P<0.05), and the protein level and activity of heparinase in lung tissue, as well as the relative expression of pro-heparinase and active heparinase were increased compared with the control group(all P<0.05). The dyspnea, hypoxemia and acute lung injury of mice in the anti-histone antibody group were alleviated, and the protein level of heparinase in lung tissue, as well as the relative expression levels of pro-heparinase and active heparinase were decreased(all P<0.05), compared with chlorine injury group and histone injury group.The dyspnea, hypoxemia and acute lung injury were alleviated in the heparinase inhibitor group, and the activity of heparinase and the relative expression of pro-heparinase in the lung tissue were decreased compared with the chlorine injury group(all P<0.05). CONCLUSION: During the occurrence and development of chlorine-induced ARDS in mice, extracellular histones aggravate lung injury by inducing the expression and activation of heparinase. Acute lung injury can be alleviated by inhibiting the expression and activation of heparinase.

Transfection Efficiency of Ad5F11p-GFP on CIK and NK-92 Cells and Its Influence on Biological Characteristics / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To study transfection efficiency of Ad5F11p-GFP and its influence on biological characteristics of CIK and NK-92 cells in order to predict the application of Ad5F11p vector in immunotherapy.</p><p><b>METHODS</b>Two kinds of immune cells, cytokine-induced killer (CIK) cells and natural-killer (NK) cell line NK-92 cells, were transfected by Ad5F11p-GFP at different multiplicity of transfection (MOI), and untransfected immune cells were used as negative control. GFP expression was determined by flow cytometry, the cell morphology was observed with microscope, the cell proliferation was analyzed by trypan blue staining, specific cytotoxicity of NK-92 cells was determined by LDH assay.</p><p><b>RESULTS</b>About 90% of transfection efficiency for NK-92 cells could be achieved at a MOI of 25, while the transfection efficiency for CIK was less than 40% at a MOI of 200. In addition, the transfection efficiency basically unchanged at the same MOI for 48 h and 96 h, and the immune cells transfected with the virus trended to form agglomeration, displaying slower proliferation, increase of IFN-γ release and enhancement of tumor killing activity.</p><p><b>CONCLUSION</b>Ad5F11p- modified NK-92 shows a good prospect for adoptive immunotherapy.</p>

Analysis of developing feature of pneumoconiosis / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To get the message about the developing feature of pneumoconiosis by analyzing the data from 353 cases of pneumoconiosis diagnosed in our hospital recent 6 years.</p><p><b>METHODS</b>To analyze the onset age, onset service years and incubation period of 353 cases of pneumoconiosis, especially in silicosis, coal worker's pneumoconiosis and potter's pneumoconiosis.</p><p><b>RESULTS</b>353 patients referred to 10 species of pneumoconiosis, the silicosis, coal worker's pneumoconiosis and potter's pneumoconiosis were accounted for 28.90%, 43.34% and 15.01% of total pneumoconiosis respectively. Diagnosed patients who began to exposed to dust during 1950's to 1980's accounted for 84.99% of all the diagnosed patients. The onset age, onset service years and incubation period of silicosis, coal worker's pneumoconiosis and potters pneumoconiosis all showed a shorten trend compared each other every decade, especially after 1980's, but excluded potter's pneumoconiosis because of that the ceramics industry switched to other products in Beijing. There was a positive correlation relationship between average onset age and incubation period in three main species of pneumoconiosis mentioned above, but no significant difference could be seen in average promotion years. Additionally, comparing with other type of work, jade machining workers showed such a characteristic as younger onset and short incubation period.</p><p><b>CONCLUSION</b>The development situation of silicosis, coal worker's pneumoconiosis is still no so optimistic, and the strict surveillance and administration especially to the township enterprises with poor production conditions should get further strengthen.</p>

Granulocyte colony-stimulating factor gene polymorphism in patients with systemic lupus erythematosus in Northern Chinese / 中华风湿病学杂志

Objective To analyze the polymorphism(s) or mutation(s) of the granulocyte colony-stim- ulating factor gene (G-CSF,Csf3) and its possible association with the susceptibility to systemic lupus erythe- matosus (SLE).Methods Polymorphism screening was carried out by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP),using DNA from 204 Northern Chinese patients with SLE,459 ethnically matched healthy control,78 patients with other autoimmune diseases.Results It was i- dentified that 4 polymorphisms at position 1869,1205,1931,and 394.The nucleotide sequences of the sample that showed different SSCP patterns and different distribution between SLE and healthy controls were deter- mined by direct sequencing.Only the distribution of the fifth extron of Csf3 (1931) between SLE and controls was different.The frequency of A/G genotype (60.5%) and A allele (53.2%) at position 1931 in patients with SLE was significantly higher than that in healthy controls (51.6% and 45.2%,respectively),whereas,the fre- quency of G/G genotype (16.2%) and G allele (46.8%) in patients with SLE was lower than that in healthy controls (28.9% and 54.8% respectively).Conclusion The results suggest that Csf3 (1931) is significantly associated with the susceptibility to SLE in Northern Chinese patients .Further population and functional stud- ies will be followed to establish Csf3 (1931) as one of the susceptible genes for SLE.